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Table 1 Callus formation from leaf segments of Piper hispidinervum under the effect of different combinations of growth regulators, α-naphtalenoacetic acid (NAA), and 6-benzylaminopurine (BAP), aiming at somatic embryogenesis, after 30 and 60 days of cultivation

From: Indirect somatic embryogenesis of Piper hispidinervum L. and evaluation of the regenerated plants by flow cytometry

  Cultivation time
NAA + BAP (mg L−1) 30 days 60 days
0.0 + 0.0 0.0 cA* 0.0 cA
5.0 + 0.0 0.0 cA 0.0 cA
10.0 + 0.0 0.0 cA 0.0 cA
0.0 + 2.5 4.0 bA 32.0 aB
5.0 + 2.5 12.0 aA 32.0 aB
10.0 + 2.5 12.0 aA 12.0 bA
Average 5.0 A 20.6 B
  1. *Means followed by the same letter (uppercase for horizontal comparison and lowercase for vertical comparison) do not differ statistically by the Scott-Knott test at 5% significance