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Table 1 Callus formation from leaf segments of Piper hispidinervum under the effect of different combinations of growth regulators, α-naphtalenoacetic acid (NAA), and 6-benzylaminopurine (BAP), aiming at somatic embryogenesis, after 30 and 60 days of cultivation

From: Indirect somatic embryogenesis of Piper hispidinervum L. and evaluation of the regenerated plants by flow cytometry

 

Cultivation time

NAA + BAP (mg L−1)

30 days

60 days

0.0 + 0.0

0.0 cA*

0.0 cA

5.0 + 0.0

0.0 cA

0.0 cA

10.0 + 0.0

0.0 cA

0.0 cA

0.0 + 2.5

4.0 bA

32.0 aB

5.0 + 2.5

12.0 aA

32.0 aB

10.0 + 2.5

12.0 aA

12.0 bA

Average

5.0 A

20.6 B

  1. *Means followed by the same letter (uppercase for horizontal comparison and lowercase for vertical comparison) do not differ statistically by the Scott-Knott test at 5% significance