Table 4 CCD of selected variables with the experimental and predicted response
Run | A: l-asparagine | B: peptone | C: glucose | D: Na2HPO4 | l-asparaginase activity (U/mL) | Residuals | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Coded | Actual | Coded | Actual | Coded | Actual | Coded | Actual | Observed | Predicted | ||
1 | 0 | 10 | 0 | 5 | 0 | 1 | 0 | 6 | 40.3819 | 42.7545 | − 2.37 |
2 | − 1 | 5 | + 1 | 7 | + 1 | 1.5 | + 1 | 8 | 29.2708 | 29.7992 | − 0.53 |
3 | − 1 | 5 | + 1 | 7 | − 1 | 0.5 | + 1 | 8 | 24.7569 | 24.6849 | 0.07 |
4 | + α | 20 | 0 | 5 | 0 | 1 | 0 | 6 | 44.2014 | 43.2235 | 0.98 |
5 | − 1 | 5 | − 1 | 3 | + 1 | 1.5 | + 1 | 8 | 23.7153 | 23.0642 | 0.65 |
6 | 0 | 10 | 0 | 5 | + α | 2 | 0 | 6 | 32.3958 | 31.911 | 0.48 |
7 | 0 | 10 | 0 | 5 | 0 | 1 | 0 | 6 | 43.1597 | 42.7545 | 0.41 |
8 | + 1 | 15 | − 1 | 3 | + 1 | 1.5 | + 1 | 8 | 38.9931 | 39.7518 | − 0.76 |
9 | + 1 | 15 | − 1 | 3 | − 1 | 0.5 | − 1 | 4 | 28.2292 | 28.2645 | − 0.04 |
10 | 0 | 10 | 0 | 5 | 0 | 1 | − α | 2 | 21.2847 | 22.3334 | − 1.05 |
11 | 0 | 10 | 0 | 5 | 0 | 1 | 0 | 6 | 42.8125 | 42.7545 | 0.06 |
12 | + 1 | 15 | − 1 | 3 | − 1 | 0.5 | + 1 | 8 | 37.6042 | 37.5889 | 0.02 |
13 | 0 | 10 | 0 | 5 | 0 | 1 | + α | 10 | 37.2569 | 36.2513 | 1.01 |
14 | + 1 | 15 | + 1 | 7 | − 1 | 0.5 | − 1 | 4 | 28.75 | 28.793 | − 0.04 |
15 | − 1 | 5 | − 1 | 3 | − 1 | 0.5 | + 1 | 8 | 19.5486 | 19.7294 | − 0.18 |
16 | − 1 | 5 | + 1 | 7 | − 1 | 0.5 | − 1 | 4 | 16.4236 | 16.2285 | 0.20 |
17 | − 1 | 5 | + 1 | 7 | + 1 | 1.5 | − 1 | 4 | 25.7986 | 25.2057 | 0.59 |
18 | + 1 | 15 | + 1 | 7 | + 1 | 1.5 | + 1 | 8 | 40.3819 | 41.2351 | − 0.85 |
19 | − 1 | 5 | − 1 | 3 | − 1 | 0.5 | − 1 | 4 | 11.9097 | 10.4484 | 1.46 |
20 | 0 | 10 | 0 | 5 | 0 | 1 | 0 | 6 | 44.2014 | 42.7545 | 1.45 |
21 | 0 | 10 | 0 | 5 | 0 | 1 | 0 | 6 | 42.8125 | 42.7545 | 0.06 |
22 | 0 | 10 | 0 | 5 | 0 | 1 | 0 | 6 | 43.1597 | 42.7545 | 0.41 |
23 | − α | 0 | 0 | 5 | 0 | 1 | 0 | 6 | 12.9514 | 13.9715 | − 1.02 |
24 | + 1 | 15 | − 1 | 3 | + 1 | 1.5 | − 1 | 4 | 34.8264 | 34.2902 | 0.54 |
25 | + 1 | 15 | + 1 | 7 | + 1 | 1.5 | − 1 | 4 | 36.2153 | 36.5982 | − 0.38 |
26 | 0 | 10 | 0 | 5 | − α | 0 | 0 | 6 | 20.2431 | 20.771 | − 0.53 |
27 | − 1 | 5 | − 1 | 3 | + 1 | 1.5 | − 1 | 4 | 17.4653 | 17.6461 | − 0.18 |
28 | 0 | 10 | − α | 1 | 0 | 1 | 0 | 6 | 24.0625 | 24.7927 | − 0.73 |
29 | 0 | 10 | + α | 9 | 0 | 1 | 0 | 6 | 32.7431 | 32.0561 | 0.69 |
30 | + 1 | 15 | + 1 | 7 | − 1 | 0.5 | + 1 | 8 | 36.9097 | 37.2927 | − 0.38 |