Isolation, molecular identification of lipid-producing Rhodotorula diobovata: optimization of lipid accumulation for biodiesel production

Journal of Genetic Engineering and Biotechnology

Table 2 Microscopic examination of lipid droplets produced by yeast isolates stained with Sudan Black B

Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection
S1	++	Pl3	+	On1	+	Ca2	–	M1	+++
S2	+	Pl4	–	On2	++	Su1	+	M2	++
S3	++	D1	–	On3	+	Su2	+	So1	+++
S4	++	D2	++	On4	–	Su3	++	So2	+
S5	–	Pe1	–	C1	+	Su4	–	So3	–
S6	+	Pe2	+	C2	++	Su5	++	To1	+
S7	+	G	++	C3	+	Su6	+	Co1	+++
S8	+	B	+	Tu1	–	Gr1	+	Co2	+
S9	–	Ol1	–	Tu2	++	Gr2	++	Co3	++
S10	–	Ol2	–	Tu3	+	Gr3	+++
S11	++	Ol3	+++	Cu1	+	Sf1	++
S12	+	Ol4	–	Cu2	–	Sf2	++
Pl1	+	Ol5	–	Cu3	–	Sf3	–
Pl2	–	Ol6	+	Ca1	+	Sf4	++

Cells containing different amounts of fat droplets were indicated by signs: −: non-lipid-producer, +: low lipid droplets, ++: moderate lipid droplets, +++: high lipid droplets

Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection
S1	++	Pl3	+	On1	+	Ca2	–	M1	+++
S2	+	Pl4	–	On2	++	Su1	+	M2	++
S3	++	D1	–	On3	+	Su2	+	So1	+++
S4	++	D2	++	On4	–	Su3	++	So2	+
S5	–	Pe1	–	C1	+	Su4	–	So3	–
S6	+	Pe2	+	C2	++	Su5	++	To1	+
S7	+	G	++	C3	+	Su6	+	Co1	+++
S8	+	B	+	Tu1	–	Gr1	+	Co2	+
S9	–	Ol1	–	Tu2	++	Gr2	++	Co3	++
S10	–	Ol2	–	Tu3	+	Gr3	+++
S11	++	Ol3	+++	Cu1	+	Sf1	++
S12	+	Ol4	–	Cu2	–	Sf2	++
Pl1	+	Ol5	–	Cu3	–	Sf3	–
Pl2	–	Ol6	+	Ca1	+	Sf4	++

Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection
S1	++	Pl3	+	On1	+	Ca2	–	M1	+++
S2	+	Pl4	–	On2	++	Su1	+	M2	++
S3	++	D1	–	On3	+	Su2	+	So1	+++
S4	++	D2	++	On4	–	Su3	++	So2	+
S5	–	Pe1	–	C1	+	Su4	–	So3	–
S6	+	Pe2	+	C2	++	Su5	++	To1	+
S7	+	G	++	C3	+	Su6	+	Co1	+++
S8	+	B	+	Tu1	–	Gr1	+	Co2	+
S9	–	Ol1	–	Tu2	++	Gr2	++	Co3	++
S10	–	Ol2	–	Tu3	+	Gr3	+++
S11	++	Ol3	+++	Cu1	+	Sf1	++
S12	+	Ol4	–	Cu2	–	Sf2	++
Pl1	+	Ol5	–	Cu3	–	Sf3	–
Pl2	–	Ol6	+	Ca1	+	Sf4	++

Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection	Isolate’s code	Lipid detection
S1	++	Pl3	+	On1	+	Ca2	–	M1	+++
S2	+	Pl4	–	On2	++	Su1	+	M2	++
S3	++	D1	–	On3	+	Su2	+	So1	+++
S4	++	D2	++	On4	–	Su3	++	So2	+
S5	–	Pe1	–	C1	+	Su4	–	So3	–
S6	+	Pe2	+	C2	++	Su5	++	To1	+
S7	+	G	++	C3	+	Su6	+	Co1	+++
S8	+	B	+	Tu1	–	Gr1	+	Co2	+
S9	–	Ol1	–	Tu2	++	Gr2	++	Co3	++
S10	–	Ol2	–	Tu3	+	Gr3	+++
S11	++	Ol3	+++	Cu1	+	Sf1	++
S12	+	Ol4	–	Cu2	–	Sf2	++
Pl1	+	Ol5	–	Cu3	–	Sf3	–
Pl2	–	Ol6	+	Ca1	+	Sf4	++