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Journal of Genetic Engineering and Biotechnology
Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean

Fig. 1

Diagram representing the vector pRicRNAi used to obtain the transgenic castor bean event TB14S-5D. The vector contains the ahas gene (ahas5′: ahas gene promoter; ahas: A. thaliana AHAS coding sequence; ahas3′: ahas gene terminator), the intron-hairpin cassette to silence ricin endogenous genes (35S-5′: 35S promoter of Cauliflower mosaic virus; pdk: Flaveria trinervia pdk intron; ocs3′: octopine synthase terminator from Agrobacterium tumefaciens, ricf: 460-bp ricin gene fragments cloned in sense and antisense orientations) and the gus gene (act2-5′: actin 2 promoter from A. thaliana; gus: β-glucuronidase coding region; nos3′: nopaline synthase terminator from A. tumefaciens)

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