Table 1 Summary of purification for alpha-amylase obtained from the submerged fermentation of P. tuber-regium
Fractions | Total Protein (mg) | Total Activity (U) | Specific Activity (U/mg) | Purification Fold | Yield (%) |
|---|---|---|---|---|---|
Crude extract | 230.74 | 610.29 | 2.65 | 1.00 | 100.00 |
Ammonium sulphate precipitation (80%) | 95.17 | 470.12 | 4.94 | 1.86 | 76.40 |
CM sephadex c25 Ion-exchange chromatography | 36.14 | 189.20 | 5.26 | 2.00 | 30.75 |