Skip to main content

Table 3 Analysis of variance (ANOVA), Fit statistic of response model, and coefficient table for LiP enzyme activity of Bacillus mycoides

From: Response surface methodology mediated optimization of Lignin peroxidase from Bacillus mycoides isolated from Simlipal Biosphere Reserve, Odisha, India

Analysis of variance (ANOVA)

Source

Sum of squares

Degree of freedom

Mean square

F value

p value

Model

2417.07

10

247.11

55.92

<0.0001

A—Incubation time

303.55

1

303.55

68.69

<0.0001

B—pH

433.32

1

433.32

98.05

<0.0001

C—Substrate conc.

18.12

1

18.12

4.10

0.0599

D—Temperature

20.40

1

20.40

4.62

0.0473

AB

50.77

1

50.77

11.49

0.0037

AC

4.48

1

4.48

1.01

0.3288

AD

93.36

1

93.36

21.13

0.0003

BC

54.06

1

54.06

12.23

0.0030

BD

984.86

1

984.86

222.86

<0.0001

CD

508.14

1

508.14

114.98

<0.0001

A2

698.77

1

698.77

87.20

<0.0001

B2

448.76

1

448.76

682.5

<0.0001

C2

831.56

1

831.56

144.55

<0.0001

D2

1.99

1

1.99

0.4321

<0.0001

Residual

95.23

16

8.08

  

Lack of fit

70.37

14

5.03

0.4522

0.9513

Pure error

0.3398

2

0.1699

  

Cor total

2541.78

26

   

Fit statistics of the response model

R2

Adjusted R2

Predicted R2

Adeq precision

C.V. %

Standard deviation

0.9722

0.9548

0.9073

32.3452

2.52

0.6194

Coefficient table for lignin peroxidase activity

Interacting terms

AB

AC

AD

BC

BD

CD

p value

0.0037

0.3288

0.0030

0.0030

<0.0001

<0.0001