Construction, expression, and in vitro assembly of virus-like particles of L1 protein of human papillomavirus type 52 in Escherichia coli BL21 DE3

Journal of Genetic Engineering and Biotechnology

Table 1 Bacterial strains and plasmids used in this study

Strains and plasmids	Characteristics	Source
E. coli Mach1™ -T1^R	Cloning host strain	Invitrogen, USA
E. coli BL21 DE3	Expression host strain	Invitrogen, USA
pD451-MR_L1-HPV52	Kan^r; T7 Promoter, cloning vector	ATUM, USA
pETSUMO	Kan^r; T7 promoter; expression vector	Invitrogen, USA