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Journal of Genetic Engineering and Biotechnology
Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats

Fig. 2

a Oral treatment of P. columbinus extract (100 mg/kg) on TNF-α in rat treated with CTX chemotherapy. Each bar is represented by a mean ± SE (n = 8). Asterisk indicates a significant difference from the control and treated with P. columbinus extract. b Agarose gel electrophoresis shows DNA fragmentation. Lane 1: M: DNA marker with 100 bp. Lane 2: control group. Lane 3: normal rats administered P. columbinus extract. Lane 3: POF model. Lane 4: CTX+P. columbinus extract. c Western blot analysis for protein expression of caspase-3 and beta actin in different studied groups. Lane 1: control group. Lane 2: normal rats administered P. columbinus extract. Lane 3: POF group. Lane 4: CTX+ P. columbinus extract. Data is expressed as mean ± SD of three independent experiments. *p < 0.05 ≠ control and P. columbinus extract groups

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