Table 1 Sequence of primers, size of the PCR products, and restriction enzyme used in genotyping of the rs7903146 and rs12255372 in the TCF7L2 gene
Polymorphism | Sequence of primers | Size of PCR product (bp) | Restriction enzyme |
|---|---|---|---|
rs7903146 (C/T) | Forward: 5′-CTGGAAACTAAGGCGTGA GG-3′ Reverse: 5′-GGGTCGATGTTGTTGAGC TT-3′ | 113 | RsaI |
rs12255372 (G/T) | Forward: 5′-GAGAGCTAAGCA CTT TTTAGG TA-3′ Reverse: 5′-CCTCATACGGCAATTAAATTATACA-3′ | 346 | Tsp509I |