Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

Journal of Genetic Engineering and Biotechnology

Table 1 The oligonucleotide primer sets designed for the amplification of the IGF2 in three populations of Japanese quails. The present annotations of this study variants were based on GenBank accession number NC_029520.1

Set	Primer code	Primer sequence (5′ → 3′)	Locus	Length	Annealing temp.
1	IGF2,exo1-F	GGTCCCTCTAGTGACACGC	Exon 1	365 bp	NA
1	IGF2,exo1-R	CAAAGCGAGGAGAGAGAGCC			NA
2	IGF2,exo2-F	TTGGCATAGCATGAGGTGGG	Exon 2	232 bp	61.0 °C
2	IGF2,exo2-R	ATGGCTTCTTTCCCCAGGTG			61.0 °C
3	IGF2,exo3-F	CTACCTTGTTGAGGGCTGGG	Exon 3	236 bp	60.7 °C
3	IGF2,exo3-R	TGGGTAAAGGGTGACGAAGC			60.7 °C
4	IGF2,exo4-F	CCGGCTGGTCACAGTTCATT	Exon 4	277 bp	59.8 °C
4	IGF2,exo4-R	GTTGTTCTCCCTTCCCCAGG			59.8 °C