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Journal of Genetic Engineering and Biotechnology
Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

Fig. 1

A schematic diagram for the IGF2 gene-based PCR-SSCP-sequencing strategy in three lines of Japanese quails. A PCR design of three PCR-specific primers pairs for the amplification of 232 bp, 236 bp, and 277 bp in exon 2, exon 3, and exon 3, respectively. B Post-PCR genotyping using SSCP technique, in which only exon 2 showed two different genotypes. C Sequencing reactions interpretation of the detected genotypes, in which only one SNP was detected in the exon 2. D Characterization of the observed SNP, in which a silent effect p.F79= was observed in the detected SNP. The positions of primers and the nomenclature of the observed SNPs are listed according to NCBI Reference Sequence: NC_029520.1 following the nomenclature described in varnomen.hgvs.org/

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