Table 1 Hydrolysis conditions for multimeric peptide cleavage
From: Expression of acid cleavable Asp-Pro linked multimeric AFP peptide in E. coli
Method | Hydrolysis conditions | Time, h |
|---|---|---|
A | 10% acetic acid, adjusted to pH 2.5 with pyridine, 37°С | 48–96 |
B | 10% acetic acid, 25% propanol-1 adjusted to pH 2.5 with pyridine, 37°С | 48–96 |
C | 50% formic acid, 37°С | 48–96 |
D | 70% formic acid, 37°С | 24–96 |
E | 90% formic acid, 37°С | 48–72 |
F | 0.1 M HCl + 12%SDS, 37°C | 24–120 |
G | 10% acetic acid + 12%SDS, 37°C | 24–120 |
H | 0.1 M HCl + 12%SDS, 95°C | 0.5–10 |
I | 10% acetic acid + 12%SDS, 95°C | 0.5–10 |
J | Tris-HCl 50 mM pH 7.0 (at RT) + 12%SDS, 95°C | 0.5–10 |
K | 10% acetic acid, adjusted to pH 2.5 with pyridine + 6M guanidine HCl, 37°C | 24–72 |
L | 0.1 M HCL + 6M guanidine HCl, 37°C | 24–72 |