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Table 1 Hydrolysis conditions for multimeric peptide cleavage

From: Expression of acid cleavable Asp-Pro linked multimeric AFP peptide in E. coli

Method Hydrolysis conditions Time, h
A 10% acetic acid, adjusted to pH 2.5 with pyridine, 37°С 48–96
B 10% acetic acid, 25% propanol-1 adjusted to pH 2.5 with pyridine, 37°С 48–96
C 50% formic acid, 37°С 48–96
D 70% formic acid, 37°С 24–96
E 90% formic acid, 37°С 48–72
F 0.1 M HCl + 12%SDS, 37°C 24–120
G 10% acetic acid + 12%SDS, 37°C 24–120
H 0.1 M HCl + 12%SDS, 95°C 0.5–10
I 10% acetic acid + 12%SDS, 95°C 0.5–10
J Tris-HCl 50 mM pH 7.0 (at RT) + 12%SDS, 95°C 0.5–10
K 10% acetic acid, adjusted to pH 2.5 with pyridine + 6M guanidine HCl, 37°C 24–72
L 0.1 M HCL + 6M guanidine HCl, 37°C 24–72