Fig. 3
From: Expression of acid cleavable Asp-Pro linked multimeric AFP peptide in E. coli
RP-HPLC separation of hydrolysis mixture (Symmetry Prep™ C18 7μm 7.8 × 300 mm). Mobile phase A—0.1% TFA in water, mobile phase B—100% AcN. 30% B isocratic followed by gradient elution from 30 to 70% AcN. Constant flow rate—3 ml/min. Detection—214 nm. The black line—hydrolysis mixture, the blue line—purified AFPpep5 multimer, and the green line—mobile phase composition