Table 1 presents the QPCR experiment steps

1

Total RNA extraction.

2

Confirm the good purity and quantity of RNA, with 260/280 nm > 1.8 and >200 ng yield by NanoDrop spectrophotometer.

3

cDNA strand Synthation using cDNA kit

4

QPCR primer design using premier 5.0 for respective genes and confirmation of their accuracy through bioinformatics UCSC software.

5

The QPCR of the cDNA was performed by using master mix probe and the previously designed and checked primers. The protocol was as follows:

95 °C for 5 min, denaturing cycles as 94 °C for 30 s, annealing as 30-s temperature (Tm), extending at 30 s for 72 °C, with a final extension of 10 min at 72 °C.

6

Control group in experiment as after normalization of mRNA level and as a control group of actin.

7

The experiment samples were conducted in three replicates.