Table 2 URP and SCoT primers and their amplification results generated in the Rosa damascena Mill. germplasm

URP

URP-1

ATCCAGGTCCGAGACAACC

48

20

20

100

0.48

16.35

9.6

URP-2

CCCAGCAACTGATCGCACAC

48

20

20

100

0.43

13.7

8.6

URP-3

AGGACTCGATAACAGGCTCC

48

20

20

100

0.44

14.1

8.8

URP-4

ATGTGTGCGATCAGTTGCTG

48

16

16

100

0.38

8.75

6.08

URP-6

GGACAAGAAGAGGATGTGGA

48

19

19

100

0.47

14.65

8.93

URP-8

CCTCCTCCCTCCT

48

20

20

100

0.39

11.65

7.8

URP-9

AGGGCTGGAGGAGGGC

48

20

20

100

0.38

11.6

7.6

URP-10

CCTGTGTGTGTGCAT

48

20

20

100

0.43

13.5

8.6

URP-11

ATGCACACACACAGG

48

18

18

100

0.40

11.85

7.2

URP-12

GGTGAAGCACAGGTG

48

18

18

100

0.44

12.5

7.92

URP-13

GGTGTAGAGAGGGGT

48

19

19

100

0.45

13.6

8.55

URP-15

GGCAGGATTGAAGC

48

20

20

100

0.43

14.35

8.6

URP-17

AGGAGGAGGGGAAGG

48

20

20

100

0.45

14.5

9

URP-18

GAGGGTGGCGGCTCT

48

19

19

100

0.40

11.65

7.6

Mean

19.21

19.21

100

0.42

13.05

8.20

SCoT

SCoT-2

CAA CAA TGG CTA CCA CCC

56

17

17

100

0.38

9.9

6.46

SCoT-3

CAA CAA TGG CTA CCA C CG

56

17

17

100

0.43

12.05

7.31

SCoT-4

CAA CAA TGG CTA CCA CCT

54

20

20

100

0.45

14.59

9

SCoT-5

CAA CAA TGG CTA CCA CGA

54

16

16

100

0.40

10.29

6.4

SCoT-8

CAA CAA TGG CTA CCA CGT

54

20

20

100

0.38

11.85

7.6

SCoT-9

CAA CAA TGG CTA CCA GCA

54

18

18

100

0.39

10.63

7.02

SCoT-11

AAG CAA TGG CTA CCA CCA

54

18

18

100

0.41

11.61

7.38

SCoT-12

ACG ACA TGG CGA CCA ACG

58

20

20

100

0.45

13.37

9

SCoT-14

ACG ACA TGG CGA CCA CCG

61

17

17

100

0.43

12.15

7.31

SCoT-15

ACG ACA TGG CGA CCG CGA

61

18

18

100

0.43

13.51

7.74

SCoT-21

CAC CAT GGC TAC CAC CAT

56

19

19

100

0.1

13.12

1.9

SCoT-26

ACA ATG GCT ACC ACC ATC

54

16

16

100

0.44

11.02

7.04

Mean

18.18

18.18

100

0.37

12.09

7.35