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Table 1 Phenotypic and molecular characteristics of Chromobacterium vialoceum strain TRFM-24

From: Characterization and low-cost preservation of Chromobacterium violaceum strain TRFM-24 isolated from Tripura state, India

Characteristics Strain TRFM-24
Colony feature after 24-h growth Growth on TSA, BHI, MacConkey agar, NA: Light purple, circular, smooth, entire, convex, size 0.5 mm and non-flourescent
KB agar: pin point colony with no pigmentation; LB agar: dark purple, circular, smooth, entire, convex, size 1.0 mm and non-flourescent
Cell shape rod
Gram reaction -ve
Catalase test +ve
Oxidase test +ve
Arginine dihydrolysis +ve
Urease hydrolysis -ve
Citrate utilization +ve
Indole production -ve
Triple sugar agar +ve for glucose fermentation
H2S production -ve
Nitrate reduction -ve
Methyl red +ve
Voges–Proskauer -ve
Starch hydrolysis +ve
Gelatin hydrolysis +ve
Cellulose hydrolysis +ve
Lipase production -ve
Anaerobic growth Poor growth with no pigment production
Haemolysis test on blood agar +ve
DNAse test +ve
Growth at different pH & temperatures Optimum growth: pH 6 to 9
No growth: below 5 and above 12 pH
Optimum growth: 30 °C
No growth: at 10 °C & 45 °C
Acid formation: sugar utilization Fully utilized: fructose and mannose
Poorly utilized: dextrose, inulin, cellobiose and xylose
Amino acid utilization Arginine, ornathine and lysine
Chemotaxonomy
  Fatty acid profiling (Sim: 0.6)
C16:1ω7c (32.7%), C16:0 (26.3%), C18:1ω7c (16.9%), C12:0 (6.2%), C10:0 (4.8%) and C12:0–3 OH (4.1%)
Molecular analysis
  16S rRNA gene sequence analysis & identity
GenBank Accession MK 841034 with similarity to C. violaceum ATCC 12472