Table 1 Numerical O.D. values recorded for the reaction between coated mouse and rat serum with HRP-conjugated anti-IgG antibodies, showing working dilutions, respective O.D. and calculated ∆ O.D
From: Cross-reaction between mouse and rat immunoglobulin G: does it matter in sandwich ELISA?
Coated serum | Dilution factor | HRP-conjugated antibody | Dilution factor | O.D. ± SD | ∆ O.D. (Homologous-heterologous serum test) |
|---|---|---|---|---|---|
Rat serum | 1:500 | Anti-rat IgG | 1:1000 | 0.977 ± 0.02 | 0.14 ± 0.03 |
Anti-mouse IgG | 0.837 ± 0.05 | ||||
Anti-rat IgG | 1:2000 | 0.798 ± 0.03 | 0.14 ±0.04 | ||
Anti-mouse IgG | 0.654 ± 0.06 | ||||
1:1000 | Anti-rat IgG | 1:1000 | 0.918 ± 0.06 | 0.14 ± 0.05 | |
Anti-mouse IgG | 0.776 ± 0.04 | ||||
Anti-rat IgG | 1:2000 | 0.744 ± 0.05 | 0.14 ±0.05 | ||
Anti-mouse IgG | 0.604 ± 0.05 | ||||
Mouse serum | 1:500 | Anti-mouse IgG | 1:1000 | 1.094 ± 0.1 | 0.522 ± 0.16 |
Anti-rat IgG | 0.571 ± 0.23 | ||||
Anti-mouse IgG | 1:2000 | 1.015 ± 0.06 | 0.618 ± 0.13 | ||
Anti-rat IgG | 0.397 ± 0.2 | ||||
1:1000 | Anti-mouse IgG | 1:1000 | 1.002 ± 0.16 | 0.539 ± 0.16 | |
Anti-rat IgG | 0.463 ± 0.17 | ||||
Anti-mouse IgG | 1:2000 | 0.941 ± 0.14 | 0.624 ± 0.14 | ||
Anti-rat IgG | 0.317 ± 0.14 |