Fig. 2
From: Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans
Verification of truncation 1. A Karyotypic verification of truncation 1 (969.925 kb position) of chromosome 4 using contour-clamped homogenous electric field (CHEF). Sc, Saccharomyces cerevisiae chromosomes used as size marker, three relevant chromosome sizes are indicated by arrows at 1532 kb, 784 kb and 577 kb respectively; P, wild type strain CAF4-2; Trn.1, chromosomes of the strain carrying truncation 1. Arrows indicated for the intact homolog and truncated version of Chr4. Truncation at 969.925 kb position (~ 970 kb) generates a 633 kb long truncated version of Chr4. It runs between 784 kb and 577 kb marker chromosomes of S. cerevisiae. B PCR verification of truncation 1 with specific primers KC49/KC102. Lane M, 1 kb DNA ladder; lane P, PCR for wild type strain (no PCR product amplified); lane Trn.1, PCR for truncation junction producing 1.5 kb PCR product (expected size)