Table 3 Transformation frequency obtained on hygromycin regime and molecular screening of putative transgenics through PCR using hpt II primers of maize obtained through Agrobacterium-mediated transformation
No. of immature embryos co-cultivated | Survival frequency at subculture stage | Survival frequency in Selection-I | Survival frequency in Selection-II | Regeneration frequency | PCR +ves for hpt II | Transformation frequency (%) |
|---|---|---|---|---|---|---|
5697 | 86.29 (4916) | 71.19 (4056) | 46.58 (2654) | 29.1 (1658) | 329 | 5.77 |