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Table 3 Transformation frequency obtained on hygromycin regime and molecular screening of putative transgenics through PCR using hpt II primers of maize obtained through Agrobacterium-mediated transformation

From: Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

No. of immature embryos co-cultivated Survival frequency at subculture stage Survival frequency in Selection-I Survival frequency in Selection-II Regeneration frequency PCR +ves for hpt II Transformation frequency (%)
5697 86.29 (4916) 71.19 (4056) 46.58 (2654) 29.1 (1658) 329 5.77
  1. Numbers in parenthesis indicate total number of calli tested