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Table 2 Survival frequency of in vitro cultures at three different stages and their frequency of regeneration in maize affected by different parameters with different variables involved in Agrobacterium-mediated transformation after co-cultivation

From: Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

Parameter Variable Subculture Selection-I Selection-II Regeneration
Optical density of bacterial culture 0.6 60.17 ± 5.13 28.59 ± 4.04 17.19 ± 3.51 8.94 ± 1.00
0.8 86.93 ± 4.50 57.41 ± 7.76 37.09 ± 4.72 23.38 ± 3.51
1 73.55 ± 9.50 46.00 ± 3.60 30.66 ± 5.29 19.55 ± 6.50
Strength of the infection medium Half MS (plain) 87.93 ± 2.00 49.48 ± 5.50 40.51 ± 3.51 21.89 ± 3.51
Full MS (plain) 83.20 ± 5.56 36.98 ± 8.08 28.11 ± 6.80 16.60 ± 3.78
LS inf 74.92 ± 6.65 23.07 ± 5.00 14.46 ± 6.11 12.30 ± 5.03
pH of the infection medium pH 5.2 50.00 ± 8.88 30.00 ± 2.64 18.88 ± 2.64 13.70 ± 2.08
pH 5.4 70.30 ± 6.65 40.61 ± 2.64 29.69 ± 5.03 18.61 ± 2.08
pH 5.8 82.57 ± 5.77 60.60 ± 8.50 39.84 ± 3.78 21.51 ± 5.03
Acetosyrengone concentration 0 μM 50.28 ± 4.16 33.71 ± 1.15 17.71 ± 3.05 9.71 ± 1.15
100 μM 84.21 ± 5.68 63.28 ± 7.00 38.59 ± 2.51 23.59 ± 2.08
200 μM 73.40 ± 2.51 56.13 ± 6.42 28.40 ± 5.50 18.86 ± 4.50
Macerozyme treatment 0.1% 57.16 ± 5.13 36.16 ± 3.78 21.33 ± 5.03 12.50 ± 3.00
0.2% 42.83 ± 4.04 20.50 ± 6.55 17.00 ± 3.46 10.66 ± 2.51
0.5% 28.83 ± 7.50 12.33 ± 6.42 10.16 ± 5.13 6.33 ± 5.03
Vacuum infiltration 0 min 83.33 ± 7.63 46.16 ± 4.17 34.16 ± 3.51 17.00 ± 2.00
10 min 61.42 ± 9.07 33.28 ± 3.78 22.71 ± 4.58 11.28 ± 1.52
15 min 58.62 ± 5.77 28.79 ± 2.08 16.72 ± 2.51 7.93 ± 1.15
Infection time 10 min 80.32 ± 7.63 54.91 ± 7.63 38.68 ± 5.50 22.78 ± 5.13
15 min 70.66 ± 5.29 44.22 ± 3.21 22.44 ± 5.13 17.11 ± 0.57
30 min 58.97 ± 5.00 33.33 ± 3.00 12.99 ± 5.03 6.32 ± 0.57
Co-cultivation period 2 days 84.00 ± 3.60 52.00 ± 9.16 36.66 ± 1.15 27.66 ± 1.52
3 days 66.33 ± 9.01 36.66 ± 8.50 23.00 ± 3.46 15.83 ± 2.51
4 days 30.65 ± 5.00 13.69 ± 1.73 15.00 ± 2.00 7.17 ± 1.00