Skip to main content

Table 3 ECM cues and gene delivery. Research papers assessing the effect of ECM cues on biomaterial-mediated gene delivery

From: Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems

Biomaterial

Cell type

Transfection system

Change in transfection efficiency

Ref.

Titanium substrates, modified with RGD-functionalised PAA brushes (N/P ratio of 20)

NIH3T3 murine fibroblasts

PEI polyplexes

In RGD-functionalised PAA brushes, luciferase expression (luminescence/mg protein) was 2.5 × 108.

In non-functionalised PAA brushes, it was 7 × 106.

[163]

Fibrin hydrogels functionalised with PEG-RGD peptides

HT-1080 human fibrosarcoma cell line and NIH3T3 murine fibroblasts

Lipoplexes

For HT-1080, in RGD-functionalised hydrogels, luciferase expression (luminescence/well/day) was 104, whereas in non-functionalised hydrogels it was 103.

For NIH3T3, in RGD-functionalised hydrogels, luciferase expression (luminescence/well/day) was 104, whereas in non-functionalised hydrogels it was less than 101.

[162]

Collagen-I-alginate hydrogels, with different collagen: alginate ratios

Human BMSCs

3D-FectIN™ (OZ Biosciences)

Decreasing the collagen: alginate ratio from 1:1 to 1:2 decreased transgene expression 1000-fold.

[14]

MAP hyaluronic acid hydrogels functionalised with RGD peptides.

Human dermal fibroblasts

jetPEI®

High RGD clustering ratio (500 and 100 μM), resulted in a cumulative luciferase expression (luminescence) higher than 2 × 107.

Low RGD clustering ratio (100 and 250 μM) resulted in a cumulative luciferase expression of 1 × 107.

[185]

Collagen I, fibronectin, laminin, collagen IV, vitronectin or ECM gel coated plates

Murine MSCs

PEI polyplexes

Highest luciferase expression (luminescence/mg protein) was 7 × 108, in fibronectin-coated plates.

Lowest luciferase expression was 2 × 106 in collagen I-coated plates.

In uncoated plates, luciferase expression was 4 × 107.

[160]

Denatured or native collagen–PLGA composite vascular stents

A10 murine vascular smooth muscle cell line

Lipofectamine™

Number of transfected cells per 200x field was 1 for native collagen and almost 20 for denatured collagen.

Supplementation of tenascin-C in native collagen substrates increased transfection, with number of transfected cells per 200x field being 14.

[186]

Fibrin hydrogels, prepared with different concentrations of fibrinogen

NIH3T3 murine fibroblasts

Lipoplexes

Highest luciferase expression (luminescence/104 cells) was 550 for concentrations of 25 mg/mL. Lowest expression was 200, for fibrinogen concentrations of 50 mg/mL.

[18]

Hyaluronic acid hydrogels functionalised with RGD peptides

Murine MSCs

PEI polyplexes

RGD concentration of 100 μM resulted in a cumulative luciferase expression (luminescence) 6 × 105.

RGD concentrations of 10 μM and 400 μM resulted in a cumulative luciferase expression (luminescence) 4.5 × 105

[161]