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Table 3 ECM cues and gene delivery. Research papers assessing the effect of ECM cues on biomaterial-mediated gene delivery

From: Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems

Biomaterial Cell type Transfection system Change in transfection efficiency Ref.
Titanium substrates, modified with RGD-functionalised PAA brushes (N/P ratio of 20) NIH3T3 murine fibroblasts PEI polyplexes In RGD-functionalised PAA brushes, luciferase expression (luminescence/mg protein) was 2.5 × 108.
In non-functionalised PAA brushes, it was 7 × 106.
[163]
Fibrin hydrogels functionalised with PEG-RGD peptides HT-1080 human fibrosarcoma cell line and NIH3T3 murine fibroblasts Lipoplexes For HT-1080, in RGD-functionalised hydrogels, luciferase expression (luminescence/well/day) was 104, whereas in non-functionalised hydrogels it was 103.
For NIH3T3, in RGD-functionalised hydrogels, luciferase expression (luminescence/well/day) was 104, whereas in non-functionalised hydrogels it was less than 101.
[162]
Collagen-I-alginate hydrogels, with different collagen: alginate ratios Human BMSCs 3D-FectIN™ (OZ Biosciences) Decreasing the collagen: alginate ratio from 1:1 to 1:2 decreased transgene expression 1000-fold. [14]
MAP hyaluronic acid hydrogels functionalised with RGD peptides. Human dermal fibroblasts jetPEI® High RGD clustering ratio (500 and 100 μM), resulted in a cumulative luciferase expression (luminescence) higher than 2 × 107.
Low RGD clustering ratio (100 and 250 μM) resulted in a cumulative luciferase expression of 1 × 107.
[185]
Collagen I, fibronectin, laminin, collagen IV, vitronectin or ECM gel coated plates Murine MSCs PEI polyplexes Highest luciferase expression (luminescence/mg protein) was 7 × 108, in fibronectin-coated plates.
Lowest luciferase expression was 2 × 106 in collagen I-coated plates.
In uncoated plates, luciferase expression was 4 × 107.
[160]
Denatured or native collagen–PLGA composite vascular stents A10 murine vascular smooth muscle cell line Lipofectamine™ Number of transfected cells per 200x field was 1 for native collagen and almost 20 for denatured collagen.
Supplementation of tenascin-C in native collagen substrates increased transfection, with number of transfected cells per 200x field being 14.
[186]
Fibrin hydrogels, prepared with different concentrations of fibrinogen NIH3T3 murine fibroblasts Lipoplexes Highest luciferase expression (luminescence/104 cells) was 550 for concentrations of 25 mg/mL. Lowest expression was 200, for fibrinogen concentrations of 50 mg/mL. [18]
Hyaluronic acid hydrogels functionalised with RGD peptides Murine MSCs PEI polyplexes RGD concentration of 100 μM resulted in a cumulative luciferase expression (luminescence) 6 × 105.
RGD concentrations of 10 μM and 400 μM resulted in a cumulative luciferase expression (luminescence) 4.5 × 105
[161]