Table 1 Microsatellite/SSR primers used for this study
No. | Primer name | Primer sequences | Repeated motif | Size range (bp) | Annealing temperature (°C) |
|---|---|---|---|---|---|
1 | MPdCIR010 | F: ACCCCGGACGTGAGGTG R: CGTCGATCTCCTCCTTTGTCTC | (GA)22 | 142–162 | 52 |
2 | MPdCIR015 | F: AGCTGGCTCCTCCCTTCTTA R: GCTCGGTTGGACTTGTTCT | (GA)15 | 144–156 | 52 |
3 | MPdCIR016 | F: AGCGGGAAATGAAAAGGTAT R:ATGAAAACGTGCCAAATGTC | (GA)14 | 142–166 | 52 |
4 | MPdCIR025 | F:GCACGAGAAGGCTTATAGT R: CCCCTCATTAGGATTCTAC | (GA)22 | 220–272 | 52 |
5 | MPdCIR032 | F: CAAATCTTTGCCGTGAG R:GGTGTGGAGTAATCATGTAGTG | (GA)19 | 302–320 | 52 |
6 | MPdCIR050 | F: CTGCCATTTCTTCTGAC R: CACCATGCACAAAAATG | (GA)21 | 172–260 | 52 |
7 | MPdCIR070 | F: CAAGACCCAAGGCTAAC R: GGAGGTGGCTTTTGTAGTAT | (GA)17 | 200–250 | 52 |
8 | MPdCIR057 | F: AAGCAGCAGCCCTTCCGTAG R: GTTCTCACTCGCCCAAAAATAC | (GA)20 | 250–310 | 52 |
9 | MPdCIR085 | F: GAGAGAGGGTGGTGTTATT R: TTCATCCAGAACCACAGTA | (GA)29 | 166–244 | 52 |
10 | MPdCIR093 | F: CCATTTATCATTCCCTCTCTTG R: CTTGGTAGCTGCGTTTCTTG | (GA)16 | 175–227 | 52 |