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Table 4 Effect of various additives on activity Aspergillus sp. DHE7 β-glucosidase

From: Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation

Metal ion/chemical reagent a Residual activity/% b
Control 100 ± 0.0
Fe2+ 107 ± 1.2
Zn2+ 145 ± 5.2
Pb2+ 85 ± 3.5
Mn+ 89 ± 3.3
Hg2+ 71 ± 1.2
Co+ 65 ± 1.9
Cu2+ 70 ± 1.3
K+ 137 ± 2.9
Ca2+ 108 ± 2.1
Mg2+ 129 ± 3.5
Na+ 111 ± 2.7
EDTA 101 ± 1.5
DMSO 125 ± 4.2
Glycerol 102 ± 3.3
β-ME 149 ± 4.6
  1. aThe concentration of all metal ion/chemical reagent tested in this study was adjusted to 5 mM
  2. bThe residual activity was evaluated via pre-incubation of the purified β-glucosidase with each chemical at 25 °C for 1 h, followed by measurements of residual activity under standard assay with p-NPG. Data are expressed as mean value ± S.D. of triplicate measurements