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Table 4 Effect of various additives on activity Aspergillus sp. DHE7 β-glucosidase

From: Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation

Metal ion/chemical reagent a

Residual activity/% b

Control

100 ± 0.0

Fe2+

107 ± 1.2

Zn2+

145 ± 5.2

Pb2+

85 ± 3.5

Mn+

89 ± 3.3

Hg2+

71 ± 1.2

Co+

65 ± 1.9

Cu2+

70 ± 1.3

K+

137 ± 2.9

Ca2+

108 ± 2.1

Mg2+

129 ± 3.5

Na+

111 ± 2.7

EDTA

101 ± 1.5

DMSO

125 ± 4.2

Glycerol

102 ± 3.3

β-ME

149 ± 4.6

  1. aThe concentration of all metal ion/chemical reagent tested in this study was adjusted to 5 mM
  2. bThe residual activity was evaluated via pre-incubation of the purified β-glucosidase with each chemical at 25 °C for 1 h, followed by measurements of residual activity under standard assay with p-NPG. Data are expressed as mean value ± S.D. of triplicate measurements