Table 3 Summary of purification profile of Aspergillus sp. DHE7 β-glucosidase
Purification step | Total activity U | Total protein (mg) | Specific activity (U/mg) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
Crude enzyme extract | 43653 ± 153 | 678 ± 5.7 | 91.3 | 100 | 1.0 |
Ethanol precipitation | 40685 ± 132 | 246 ± 3.8 | 165.4 | 93.2 | 1.8 |
Dialysis | 36974 ± 145 | 213 ± 2.1 | 173.6 | 84.7 | 1.9 |
DEAE-Sephadex A-50 | 28505 ± 105 | 22.3 ± 0.8 | 1278.3 | 65.3 | 14.0 |
Sephadex G-100 | 14731 ± 127 | 3.8 ± 0.1 | 3876.6 | 33.7 | 42.5 |