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Table 3 Summary of purification profile of Aspergillus sp. DHE7 β-glucosidase

From: Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation

Purification step Total activity
U
Total protein
(mg)
Specific activity
(U/mg)
Recovery
(%)
Purification
fold
Crude enzyme extract 43653 ± 153 678 ± 5.7 91.3 100 1.0
Ethanol precipitation 40685 ± 132 246 ± 3.8 165.4 93.2 1.8
Dialysis 36974 ± 145 213 ± 2.1 173.6 84.7 1.9
DEAE-Sephadex A-50 28505 ± 105 22.3 ± 0.8 1278.3 65.3 14.0
Sephadex G-100 14731 ± 127 3.8 ± 0.1 3876.6 33.7 42.5
  1. Data are expressed as mean value ± S.D. of triplicate measurements