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Table 2 Effect of auxins supplemented MS medium on induction of roots from the shoots of Andrographis alata

From: Rapid plant regeneration, analysis of genetic fidelity, and neoandrographolide content of micropropagated plants of Andrographis alata (Vahl) Nees

Growth regulator

Concentration (μM)

Percentage of response

Mean number of roots per shoot

Average root length (cm)

MS half strength medium + auxins

 Control

0

0

0 ± 0e

0 ± 0e

 NAA

1

0

0 ± 0e

0 ± 0e

2

0

0 ± 0e

0 ± 0e

5

0

0 ± 0e

0 ± 0e

10

0

0 ± 0e

0 ± 0e

 IBA

1

100

1.4 ± 0.16c

2.10 ± 0.07b

2

100

5.6 ± 0.16b

1.48 ± 0.03c

5

100

12.20 ± 1.0a

2.18 ± 0.07b

10

16.66

1 ± 0.66cd

0.30 ± 0.04d

 IAA

1

100

2 ± 0c

2.19 ± 0.07b

2

100

2 ± 0.21c

2.80 ± 0.09a

5

0

0 ± 0e

0 ± 0e

10

0

0 ± 0e

0 ± 0e

MS quarter strength medium + auxins

 Control

0.00

0

0 ± 0g

0 ± 0h

 NAA

1

100

4 ± 0.21ef

2.34 ± 0.04c

2

100

11.80 ± 0.64b

2.42 ± 0.10c

5

66.66

11.50 ± 0.42b

1.25 ± 0.10g

10

66.66

12.33 ± 0.42b

1.30 ± 0.16fg

 IBA

1

100

19.0 ± 1.65a

3.06 ± 0.07ab

2

100

11.60 ± 0.54b

2.96 ± 0.10b

5

100

4.80 ± 0.13de

3.36 ± 0.11a

10

100

8.0 ± 0.63c

2.80 ± 0.13b

 IAA

1

66.66

4.25 ± 0.16de

1.85 ± 0.10de

2

100

6.20 ± ±0.48cd

2.10 ± 0.07cd

5

100

11.40 ± 0.80b

1.20 ± 0.20g

10

66.66

2.0 ± 0f

1.62 ± 0.13ef

  1. Data was recorded after 4 weeks of culture. Each value represents the mean ± standard error. Treatment means followed by different letters in their superscript are significantly different from each other (p = 0.05) according to Duncan’s multiple range test