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Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Sodium antiporters of Pseudomonas aeruginosa in challenging conditions: effects on growth, biofilm formation, and swarming motility

Fig. 3

Biofilm index comparison of P. aeruginosa mutants to wild-type strain (WT) grown with 0–500 mM added NaCl in stationary phase. After 24 h of incubation at pH 6.5 (panel a), pH 7.5 (panel b), and pH 8.5 (panel c), optical density of the cultures in the wells was measured at 595 nm. Then, the biofilms in the wells was stained and the wells measured at 570 nm. The biofilm index was calculated as OD570/OD595 to account for growth differences. Columns indicate mean ratio of five independent experiments, and error bars indicate standard error margins of the mean (SEM). Asterisks indicate statistically significant differences in Biofilm Index of the MrpA mutant with the addition of ≥ 400 mM NaCl at pH 7.5 (panel b) or with the addition of ≥ 200 mM NaCl at pH 8.5 (panel c) to WT with p values equal or smaller than 0.001

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