Fig. 2

Comparison of P. aeruginosa mutants to wild-type strain (WT) grown with 0-500 mM added NaCl in early exponential phase (panels a–c) and early stationary phase (panels d–f). After 6 h of incubation at pH 6.5 (panel a), pH 7.5 (panel b), and pH 8.5 (panel c) and after 18 h of incubation at pH 6.5 (panel d), pH 7.5 (panel e), and pH 8.5 (panel f), optical density at 595 nm was measured. Columns indicate mean growth of three independent experiments of each two technical replicates, and error bars indicate standard error margin of the mean (SEM). Asterisks indicate statistically significant differences of the MrpA mutant to WT with the addition of ≥ 200 mM NaCl (panel b) or ≥ 400 mM NaCl (panel d) at pH 7.5 (panel b) and the addition of ≥ 100 mM NaCl at pH 8.5 (panels c, f) with p values smaller than 0.0001