Table 3 Effect of various inhibitors on Egyptian honey bee venom PLA2
Reagent | Concentration (mM) | Inhibition % |
|---|---|---|
Control | ----- | 0.0 |
β-Mercaptoethanol | 5.0 | 27.5 |
1,10 Phenanthroline | 5.0 | 18.8 |
NaN3 | 5.0 | 73.4 |
Iodoacetic acid | 5.0 | 5.7 |
N-Methylmaleimide | 5.0 | 37.4 |
DL-Dithiothreitol (DTT) | 5.0 | 22.5 |
EDTA | 5.0 | 33.2 |
Phenylmethylsulfonylfluoride (PMSF) | 5.0 | 39.5 |