Table 2 The values of a master mix components
Component | Volume (μl) | Final concentration |
|---|---|---|
DNA sample template | 2.0 | 50 ng |
10× PCR reaction buffer | 2.0 | 1× |
Forward primer | 1.5 | 1 pmol |
Reverse primer | 1.5 | 1 pmol |
dNTPs | 2.0 | 200 μM |
TaqDNA polymerase | X | 1 U |
Sterile double-distilled water | X | Up to 20 μl |