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Table 2 The values of a master mix components

From: Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Component Volume (μl) Final concentration
DNA sample template 2.0 50 ng
10× PCR reaction buffer 2.0
Forward primer 1.5 1 pmol
Reverse primer 1.5 1 pmol
dNTPs 2.0 200 μM
TaqDNA polymerase X 1 U
Sterile double-distilled water X Up to 20 μl