Table 1 Primer pairs used to amplify study genes
Primer name | Genomic location (bp) | Sequence (5′-3′) | GC content (%) | Annealing temp. | Product size (bp) |
|---|---|---|---|---|---|
ND1 F ND1 R | 3245-3264 4370-4351 | CCCCGGTAATCGCATAAAACT TTTTGGATTCTCAGGGATGG | 45 45 | 66oC 66oC | 1125 |
ND2 F ND2 R | 4382-4402 5565-5545 | CCTATCACACCCCATCCTAAA TGCAACTTACTGAGGGCTTTG | 47 47 | 69oC 69oC | 1183 |
ATPase6 F ATPase6 R | 8531-8552 9185-9206 | AAC GAA AAT CTG TTC GCT TCA ATG TGT TG T CGT GCA GGT GA | 47 47 | 69oC 69oC | 675 |