Table 1 Primer pairs used to amplify study genes
| Primer name | Genomic location (bp) | Sequence (5′-3′) | GC content (%) | Annealing temp. | Product size (bp) |
|---|---|---|---|---|---|
|
ND1 F ND1 R |
3245-3264 4370-4351 |
CCCCGGTAATCGCATAAAACT TTTTGGATTCTCAGGGATGG |
45 45 |
66oC 66oC | 1125 |
|
ND2 F ND2 R |
4382-4402 5565-5545 |
CCTATCACACCCCATCCTAAA TGCAACTTACTGAGGGCTTTG |
47 47 |
69oC 69oC | 1183 |
|
ATPase6 F ATPase6 R |
8531-8552 9185-9206 |
AAC GAA AAT CTG TTC GCT TCA ATG TGT TG T CGT GCA GGT GA |
47 47 |
69oC 69oC | 675 |