Fig. 3
From: Paper-based PCR method development, validation and application for microbial detection
Annealing temperature optimization. a 16s r-DNA amplification (S. aureus CS): lane 1—100 bp ladder, lane 2—55 °C, lane 3—57 °C, lane 4—58 °C, lane 5—negative control. b β-galactocidase amplification using LacZ primers (E. coli. CS): lane 1—100 bp ladder, lane 2—55 °C, lane 3—57 °C, lane 4—58 °C, lane 5—negative control. c ITS amplification using ITS primers (C. albicans CS): lane 1—100bp ladder, lane 2—55 °C, lane 3—57 °C, lane 4—58 °C, lane 5—negative control. (L): 100 bp ladder