Table 5 Analysis of variance (ANOVA) for the quadratic model of xylanase enzyme activity as per Box-Behnken design

Model

22,859.79

14

1632.84

230.17

< 0.0001

A-Incubation time

704.11

1

704.11

99.25

< 0.0001

B-pH

448.96

1

448.96

63.29

< 0.0001

C-Substrate Conc.

1692.19

1

1692.19

238.53

< 0.0001

D-Temperature

1559.06

1

1559.06

219.77

< 0.0001

AB

1242.56

1

1242.56

175.15

< 0.0001

AC

1866.24

1

1866.24

263.07

< 0.0001

AD

520.30

1

520.30

73.34

< 0.0001

BC

6724.00

1

6724.00

947.82

< 0.0001

BD

879.12

1

879.12

123.92

< 0.0001

CD

208.80

1

208.80

29.43

0.0002

A²

688.87

1

688.87

97.10

< 0.0001

B²

5548.72

1

5548.72

782.15

< 0.0001

C²

1731.36

1

1731.36

244.05

< 0.0001

D²

2.98

1

2.98

0.4201

0.5291

Residual

85.13

12

7.09

Lack of fit

55.68

10

5.57

0.3782

0.8803

Pure Error

29.45

2

14.72

Cor Total

22,944.92

26