Table 4 Effect of metal ions and additives on β-galactosidase activity
Metal ions (chloride salt, 10 mM) | Relative activity (%) | Additives (1 mM) | Relative activity (%) |
|---|---|---|---|
Na+ | 98 ± 1.5 | Sodium azide | 96 ± 1 |
K+ | 100 ± 0.88 | 8-Hydroxyquinoline | 91.3 ± 1.5 |
Mg+2 | 122 ± 1 | EDTA | 55.3 ± 1.1 |
Mn+2 | 116 ± 1.7 | SDS | 62 ± 1 |
Zn+2 | 83 ± 2.1 | DTT | 100 ± 0.88 |
Ca+2 | 97 ± 1.1 | β-Mercabtoethanol | 82.5 ± 1.3 |
Ba+2 | 42 ± 1.5 | Hydroxylamine | 93 ± 0.87 |
Li+2 | 45.7 ± 1.3 | ||
Cu+2 | 8.2 ± 1 | ||
Co+2 | 39.5 ± 1.6 | ||
Cd+2 | 98 ± 2.9 | ||
Cr+3 | 82 ± 0.84 | ||
Ni+2 | 74.9 ± 1.6 | ||
Fe+3 | 22.9 ± 1.4 |