Table 3 Purification scheme for β-galactosidase from E. coli M-KH-UV-Tra44
Purification step | Total protein (mg/ml) | Total activity (U/ml) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
Crude enzyme | 4.86 ± 0.17 | 251.7 ± 1.2 | 51.7 ± 1.3 | 100 | 1 |
Ammonium sulfate (60–80%) | 1.28 ± 0.14 | 204 ± 1.6 | 159.3 ± 0.81 | 81.2 | 3.07 |
Sephadex G-100 | 0.66 ± 0.11 | 167 ± 0.82 | 253 ± 0.11 | 66.3 | 4.96 |