Table 2 Effect of some metal ions, chelating agents, and some inhibitors/activators on the partially purified NYSO laccase
Relative activity (%) (mean ± SD) | ||
|---|---|---|
Tested | Metal ion concentration | |
1 mM | 10 mM | |
Control | 100 ± 0 | 100 ± 0 |
MgCl2 | 157.8 ± 0.76 | 127.8 ± 0.75 |
CrCl2 | 169.2 ± 2.25 | 148.7 ± 1.52 |
MnCl2 | 140.1 ± 0.76 | 151.9 ± 1.70 |
HgCl2 | 60.1 ± 0.76 | 59.9 ± 0.66 |
CuSO4.5H2O | 171.1 ± 0.89 | 241.8 ± 2.02 |
FeSO4.7.H2O | 15.6 ± 0.58 | 10.9 ± 0.85 |
NiSO4 | 141.2 ± 1.10 | 198.5 ± 1.25 |
CdSO4 | 167.9 ± 1.76 | 166.5 ± 1.36 |
ZnSO4.7H2O | 177.4 ± 2.16 | 171.8 ± 2.01 |
CoSO4 | 150.6 ± 0.65 | 191.3 ± 1.42 |
Chelating agent concentration | ||
1 mM | 10 mM | |
EDTA | 215.6 ± 1.25 | 24.6 ± 0.53 |
Sodium citrate | 114.7 ± 0.3 | 99.4 ± 0.51 |
Sodium oxalate | 103.6 ± 2.36 | 95.9 ± 1.79 |
Inhibitor/activator concentration | ||
1 mM | 10 mM | |
Sodium azide | 69.0 ± 0.10 | 20.5 ± 0.50 |
DTT | 7.8 ± 2.02 | 5.5 ± 0.47 |
Urea | 157.3 ± 2.33 | 127. ±1.67 |
Imidazole | 140.7 ± 1.04 | 125.4 ± 1.58 |
Kojic acid | 125.3 ± 2.0 | 71.1 ± 1.27 |
Cysteine-HCl | 9.3 ± 1.10 | 2.1 ± 0.1 |
β-Mercaptoethanol | 7.4 ± 0.52 | 4.6 ± 0.5 |
Thioglycolic acid | 11.4 ± 1.26 | 9.6 ± 0.5 |
PMSF | 106.2 ± 1.04 | 30.1 ± 0.6 |
H2O2 | 100.8 ± 2.25 | 89.5 ± 0.5 |
Triton X-100 | 140.0 ± 0.95 | 116.2 ± 1.31 |
SDS | 2.9 ± 0.1 | 0.60 ± 0.045 |