Table 1 Primers used in this study
From: Simple innovative adaptor to improve genome walking with convenient PCR
Primers name | Primer sequence (5′-3′) | Location | Primer application | Tm (°C) |
|---|---|---|---|---|
GSP1 | GCATAACTTGTGAGATTGAGG | map30 gene | Reverse primer in the 1st PCR | 56.5 |
AP1 | GTAATACGACTCACTATACGGC | Adaptor | Adaptor primer in the 1st PCR | 56.5 |
GSP2 | GGCTAAATGGAAGAGTCG | map30 gene | Reverse primer in the 2nd PCR | 54 |
AP2 | GACTCACTATACGGCTCT | Adaptor | Adaptor primer in the 2nd PCR | 54 |
F helper | GCATGGTGAAATGCTTACTAC | map30 gene | Forward primer in the 3rd PCR | 50.5 |