Skip to main content

Table 1 Primers used in this study

From: Simple innovative adaptor to improve genome walking with convenient PCR

Primers name

Primer sequence (5′-3′)

Location

Primer application

Tm (°C)

GSP1

GCATAACTTGTGAGATTGAGG

map30 gene

Reverse primer in the 1st PCR

56.5

AP1

GTAATACGACTCACTATACGGC

Adaptor

Adaptor primer in the 1st PCR

56.5

GSP2

GGCTAAATGGAAGAGTCG

map30 gene

Reverse primer in the 2nd PCR

54

AP2

GACTCACTATACGGCTCT

Adaptor

Adaptor primer in the 2nd PCR

54

F helper

GCATGGTGAAATGCTTACTAC

map30 gene

Forward primer in the 3rd PCR

50.5