Fig. 3
From: A polyclonal antibody against a recombinantly expressed Triticum aestivum RHT-D1A protein
Purification of the rabbit anti-RHT-D1A polyclonal antibody and a western blot assay. a Purification of the rabbit anti-RHT-D1A polyclonal antibody. M: standard protein molecular weight markers; lane 1: immune serum (3 μg of protein); lane 2: the eluate from protein A–agarose (3 μg of protein). b SDS-PAGE of affinity-purified recombinant protein 6xHis-RHT-D1A. c The western blot assay. Lane 1: 3 μg of protein; lane 2: 1 μg of protein