Table 2 List of ISSR, SRAP and ISTR primer combinations obtained from the current investigation
Primer name | Sequence | Primer name | Sequence | |||
HB1 | (CAA)5 | HB 12 | (CAC)3 GC | |||
HB 2 | (CAG) 5 | HB 13 | (GAG)3 GC | |||
HB 4 | (GACA) 4 | HB 15 | (GTG) 3 GC | |||
HB 8 | (GA)6 GG | 807 | (AG)8 T | |||
HB 9 | (GT) 6 GG | 814 | (CT) 8TG | |||
HB 10 | (GA) 6 CC | 844A | (CT)8 AC | |||
HB 11 | (GT) 6 CC | 844B | (CT) 8 GC | |||
17899B | (CA) 6 GG | |||||
Marker type | Marker Name | Forward primer | Reverse primer | Marker Name | Forward primer | Reverse primer |
SRAP | Em 1R/DN 6 F | GACTGCGTACGAATTAAT | /TGAGTCCAAACCGGTAA | Em 10 R/DN 8 F | GACTGCGTACGAATTCAT | TGAGTCCAAACCGGTGC |
Em 1R/DN 7 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTCC | Em 10 R/DN 9 F | GACTGCGTACGAATTCAT | TGAGTCCAAACCGGTCA | |
Em 1R/DN 8 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTGC | Em 10 R/DN 10 F | GACTGCGTACGAATTCAT | TGAGTCCAAACCGGGCT | |
Em 1R/DN 9 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTCA | Em 10 R/DN 12 F | GACTGCGTACGAATTCAT | TGAGTCCAAACCGGTGT | |
Em 1R/DN 10 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGGCT | Em 12 R/DN 11 F | GACTGCGTACGAATTCTC | TGAGTCCAAACCGGTAG | |
Em 1R/DN 11 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTAG | Em 15 R/DN 6 F | GACTGCGTACGAATTGTC | TGAGTCCAAACCGGTAA | |
Em 1R/DN 12 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTGT | Em 15 R/DN 8 F | GACTGCGTACGAATTGTC | TGAGTCCAAACCGGTGC | |
Em 1R/DN 6 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTAA | Em 15 R/DN 9 F | GACTGCGTACGAATTGTC | TGAGTCCAAACCGGTCA | |
Em 1R/DN 7 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTCC | Em 15 R/DN 10 F | GACTGCGTACGAATTGTC | TGAGTCCAAACCGGGCT | |
Em 1R/DN 8 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTGC | Em 15 R/DN 12 F | GACTGCGTACGAATTGTC | TGAGTCCAAACCGGTGT | |
Em 1R/DN 9 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTCA | Em 18 R/DN 12 F | GACTGCGTACGAATTGAG | TGAGTCCAAACCGGTGT | |
Em 1R/DN 10 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGGCT | Em 20 R/DN 6 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGTAA | |
Em 1R/DN 11 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTAG | Em 20 R/DN 7 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGTCC | |
Em 1R/DN 12 F | GACTGCGTACGAATTAAT | TGAGTCCAAACCGGTGT | Em 20 R/DN 8 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGTGC | |
Em 6 R/DN 7 F | GACTGCGTACGAATTGCA | TGAGTCCAAACCGGTCC | Em 20 R/DN 9 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGTCA | |
Em 8 R/DN 8 F | GACTGCGTACGAATTCTG | TGAGTCCAAACCGGTGC | Em 20 R/DN 10 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGGCT | |
Em 9 R/DN 9 F | GACTGCGTACGAATTCAG | TGAGTCCAAACCGGTCA | Em 20 R/DN 11 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGTAG | |
Em 10 R/DN 6 F | GACTGCGTACGAATTCAT | TGAGTCCAAACCGGTAA | Em 20 R/DN 12 F | GACTGCGTACGAATTGCC | TGAGTCCAAACCGGTGT | |
Em 10 R/DN 7 F | GACTGCGTACGAATTCAT | TGAGTCCAAACCGGTCC | ||||
ISTR | F1/B2 | AGGAGGTGAATACCTTAG | GGATATCCTATGAATCAAGC | F3/B2 | GTCGACATGCCATCTTTC | GGATATCCTATGAATCAAGC |
F1/B3 | AGGAGGTGAATACCTTAG | ATTCCCATCTGCACCAAT | F3/B3 | GTCGACATGCCATCTTTC | ATTCCCATCTGCACCAAT | |
F3/B5 | GTCGACATGCCATCTTTC | CTTCTGTGAAAGTCCTAG | F3/B6 | GTCGACATGCCATCTTTC | ATATATGGACTTAAGCAAGCA | |
F3/B8 | GTCGACATGCCATCTTTC | CCTCCTTATTGGGAATGATAT | F7/B3 | TGCTAGGACTTTCACAGA | ATTCCCATCTGCACCAAT | |
F3/B10 | GTCGACATGCCATCTTTC | GACCCTTTTGAAAACACATG | F7/B6 | TGCTAGGACTTTCACAGA | ATATATGGACTTAAGCAAGCA | |
F5/B7 | ATATATGGACTTAAGCAAGC | GGAATATCATTCCCAATAAG | F9/B2 | ATATGGACTTAAGCAAGCCA | GGATATCCTATGAATCAAGC | |
F5/B8 | ATATATGGACTTAAGCAAGC | CCTCCTTATTGGGAATGATAT | F9/B3 | ATATGGACTTAAGCAAGCCA | ATTCCCATCTGCACCAAT | |
F10/B6 | GGAATATCATTCCCAATAAG | ATATATGGACTTAAGCAAGCA | F9/B6 | ATATGGACTTAAGCAAGCCA | ATATATGGACTTAAGCAAGCA | |
F1/B8 | AGGAGGTGAATACCTTAG | CCTCCTTATTGGGAATGATAT | F9/B10 | ATATGGACTTAAGCAAGCCA | GACCCTTTTGAAAACACATG | |