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Journal of Genetic Engineering and Biotechnology
Fig. 6 | Journal of Genetic Engineering and Biotechnology

Fig. 6

From: Loop-mediated isothermal amplification: a rapid molecular technique for early diagnosis of Pseudomonas syringae pv. syringae of stone fruits

Fig. 6

The comparison of gel-free and gel-based SYBER Gold staining: the upper panel. Part A: lane 1, 1 kb DNA ladder; lane 2, LAMP product without dilution 10 (1 ng/μl); lane 3, LAMP product diluted by 10− 1 (0.1 ng/μl); lane 4, LAMP product diluted by 10− 2 (0.01 ng/μl); lane 5, LAMP product diluted by 10− 3 (0.001 ng/μl); lane 6, LAMP product diluted by 10− 4 (0.0001 ng/μl); and lane 7, LAMP product diluted by 10− 5 (0.00001 ng/μl). Part B: the upper row. Sample 1, a mixture of 10 μl LAMP product (X) and 10 μl SYBR Gold. Sample 2, a mixture of 10 μl 1/10 LAMP product and SYBR Gold 3. A mixture of 10 μl 1/100 LAMP product and SYBR Gold. Sample 3: a mixture of 10 μl 1/1000 LAMP product and SYBR Gold. Sample 4, 10 μl 1/10000 LAMP product and SYBR Gold. Sample 5, 10 μl of 1/100000 LAMP product. Sample 6, 10 μl of 1/1000000 LAMP product and SYBR Gold. Sample 7, 10 μl of 1/1000000 LAMP product. Part B: the lower row, 20 μl SYBR Gold

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