Fig. 5

The comparison of gel-free and gel-based SYBER Gold staining: the upper panel. Part A: lane 1, PCR product without dilution 10 (200 ng/μl); lane 2, PCR product diluted by10^− 1 (20 ng/μl); lane 3, PCR product diluted by 10^− 2 (2 ng/μl); lane 4, PCR product diluted by 10^− 3 (0.2 ng/μl); lane 5, PCR product diluted by 10^− 4 (0.02 ng/μl); and lane 6, 1 kb DNA ladder. Part B: the upper row. Sample 1, a mixture of 10 μl PCR product (X) and 10 μl SYBR Gold. Sample 2, the mixture of 10 μl X/10 PCR product and SYBR Gold. Sample 3, the mixture of 10 μl X/10 PCR product and SYBR Gold. Sample 4, 10 μl X/10000 PCR product and SYBR Gold. Sample 5, 10 μl of X100000 PCR product and SYBR Gold. The lower row, 20 μl SYBR Gold