Table 2 Biochemical properties of cold-adapted proteases (published from 2010 onwards)

Acinetobacter sp.

Serine peptidase

PMSF, EDTA, EGTA, 2-ME, Pepstatin A, DTT, and Hg²⁺

Ca²⁺, Mn²⁺, Na⁺, Zn²⁺

40/9

[63]

Bacillus sp.

Metalloprotease

Cu²⁺, Zn²⁺, Hg²⁺, EDTA, and SDS

Mg²⁺and Ca²⁺

40/7.4

[12]

Bacillus sp.

Metalloprotease

EDTA, EGTA, Ca²⁺, Cu²⁺, Mg²⁺, K⁺, Zn²⁺

Mn²⁺

20/9

[34]

Bacillus subtilis

Serine protease

Mg²⁺, Pb²⁺, Mn²⁺, Al³⁺, and Fe²⁺

Cu²⁺ and Ca²⁺

15/10

[30]

Chryseobacterium sp.

Serine protease

Butanol, acetonitrile, isopropanol, ethyl acetate, tetrahydrofuran, Hg²⁺, Zn²⁺, and Cu²⁺

Na⁺, Ca²⁺, Ba²⁺, and Fe²⁺

10/7.0–8.0

[32]

Geomyces pannorum

Aspartic protease

Methanol, isopropanol, DMSO, Mg²⁺, Fe²⁺, Ca²⁺, and Zn²⁺

Co²⁺, Mn²⁺, Cu²⁺, and Ni²⁺

60/3.0

[15]

Halobacillus sp.

Thermolysin-like protease

EGTA, Cu²⁺, EDTA, 1,10-phenanthroline, and Ni²⁺

Mn²⁺, Ca²⁺, Mg²⁺, and Ba²⁺

30/8

[37]

Lysobacter sp.

Serine peptidase

PMSF, EDTA, and Zn²⁺

Ca²⁺, Mg²⁺, Ba²⁺, Na⁺, NH₄⁺, and isopropyl alcohol

40/9.0

[18]

Penicillium nalgiovense

Serine protease

PMSF, SDS, Mn²⁺, and Zn²⁺

Ca²⁺ and Mg²⁺

35/8

[40]

Planococcus sp.

Serine protease

PMSF, DEPC, EDAC, urea, SDS, EDTA, Co²⁺, Zn²⁺, Fe³⁺, and Ni²⁺

Ca²⁺

35/10

[23]

Planococcus sp.

Serine protease

EDTA, PMSF, TNBS, EDAC, Cu²⁺, and Ni²⁺

Fe³⁺ and Ca²⁺

35/10

[60]

Planomicrobium sp.

Serine protease

PMSF and AEBSF

Ca²⁺ and Mn²⁺

35/9

[11]

Pseudomonas aeruginosa

Serine protease

PMSF and Ag⁺

Mg²⁺, K⁺, Ca²⁺, Ba²⁺, and Zn²⁺

25/10

[21]

Pseudoalteromonas sp.

Serine protease

PMSF, SDS, and H₂O₂,

Nm

25–35/8–9

[16]

Pseudoalteromonas arctica

Subtilisin-like protease

Linear alkylbenzene sulfonate (LAS) and SDS

Ca²⁺

30/9.0

[64]

Pseudomonas lundensis

Metalloprotease

EDTA, EGTA, Cu²⁺, Fe³⁺, Al³⁺, Fe²⁺, Mn²⁺, Al³⁺, and Co²⁺

Na⁺, K⁺, and Li⁺

30/10.4

[19]

Serratia marcescens

Metalloprotease

EDTA, MnCl₂, CaCl₂, CoSo₄, HgCl₂, and Na₂

Nm

40/8

[27]

Sporobolomyces roseus

Aspartic protease

2-Mercaptoethanol, dithiothreitol, SDS, and Pepstatin A

Nm

50/4

[31]

Stenotrophomonas sp.

Alkaline protease

Zn²⁺, Cu²⁺, and Co²⁺

Mg²⁺, Mn²⁺, and Ca²⁺

15/10

[28]

Stenotrophomonas maltophilia

Alkaline protease

Co²⁺

Cu²⁺, Cr²⁺

20/10

[29]