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Table 2 Biochemical properties of cold-adapted proteases (published from 2010 onwards)

From: Adaptation, production, and biotechnological potential of cold-adapted proteases from psychrophiles and psychrotrophs: recent overview

Microbial source Protease type Strong inhibitors Stimulators/enhancers Optimum temp./pH Reference
Acinetobacter sp. Serine peptidase PMSF, EDTA, EGTA, 2-ME, Pepstatin A, DTT, and Hg2+ Ca2+, Mn2+, Na+, Zn2+ 40/9 [63]
Bacillus sp. Metalloprotease Cu2+, Zn2+, Hg2+, EDTA, and SDS Mg2+and Ca2+ 40/7.4 [12]
Bacillus sp. Metalloprotease EDTA, EGTA, Ca2+, Cu2+, Mg2+, K+, Zn2+ Mn2+ 20/9 [34]
Bacillus subtilis Serine protease Mg2+, Pb2+, Mn2+, Al3+, and Fe2+ Cu2+ and Ca2+ 15/10 [30]
Chryseobacterium sp. Serine protease Butanol, acetonitrile, isopropanol, ethyl acetate, tetrahydrofuran, Hg2+, Zn2+, and Cu2+ Na+, Ca2+, Ba2+, and Fe2+ 10/7.0–8.0 [32]
Geomyces pannorum Aspartic protease Methanol, isopropanol, DMSO, Mg2+, Fe2+, Ca2+, and Zn2+ Co2+, Mn2+, Cu2+, and Ni2+ 60/3.0 [15]
Halobacillus sp. Thermolysin-like protease EGTA, Cu2+, EDTA, 1,10-phenanthroline, and Ni2+ Mn2+, Ca2+, Mg2+, and Ba2+ 30/8 [37]
Lysobacter sp. Serine peptidase PMSF, EDTA, and Zn2+ Ca2+, Mg2+, Ba2+, Na+, NH4+, and isopropyl alcohol 40/9.0 [18]
Penicillium nalgiovense Serine protease PMSF, SDS, Mn2+, and Zn2+ Ca2+ and Mg2+ 35/8 [40]
Planococcus sp. Serine protease PMSF, DEPC, EDAC, urea, SDS, EDTA, Co2+, Zn2+, Fe3+, and Ni2+ Ca2+ 35/10 [23]
Planococcus sp. Serine protease EDTA, PMSF, TNBS, EDAC, Cu2+, and Ni2+ Fe3+ and Ca2+ 35/10 [60]
Planomicrobium sp. Serine protease PMSF and AEBSF Ca2+ and Mn2+ 35/9 [11]
Pseudomonas aeruginosa Serine protease PMSF and Ag+ Mg2+, K+, Ca2+, Ba2+, and Zn2+ 25/10 [21]
Pseudoalteromonas sp. Serine protease PMSF, SDS, and H2O2, Nm 25–35/8–9 [16]
Pseudoalteromonas arctica Subtilisin-like protease Linear alkylbenzene sulfonate (LAS) and SDS Ca2+ 30/9.0 [64]
Pseudomonas lundensis Metalloprotease EDTA, EGTA, Cu2+, Fe3+, Al3+, Fe2+, Mn2+, Al3+, and Co2+ Na+, K+, and Li+ 30/10.4 [19]
Serratia marcescens Metalloprotease EDTA, MnCl2, CaCl2, CoSo4, HgCl2, and Na2 Nm 40/8 [27]
Sporobolomyces roseus Aspartic protease 2-Mercaptoethanol, dithiothreitol, SDS, and Pepstatin A Nm 50/4 [31]
Stenotrophomonas sp. Alkaline protease Zn2+, Cu2+, and Co2+ Mg2+, Mn2+, and Ca2+ 15/10 [28]
Stenotrophomonas maltophilia Alkaline protease Co2+ Cu2+, Cr2+ 20/10 [29]
  1. Nm not mentioned