In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate

Journal of Genetic Engineering and Biotechnology

Table 1 Effect of Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA₃) and 1.08 μM α-naphthaleneacetic acid (NAA) in addition to 6-benzyl adenine (BA) or kinetin (Kin) on the in vitro establishment of Silene schimperiana shoot tip and stem nodal segment explants. All treatments gave 100% of both survived explants and growth formation

Cytokinin conc. (μM)		Shoot tip		Stem nodal segment
BA	Kin	Mean no. of axillary shoots/explant	Mean length of axillary shoots (cm)	Mean no. of axillary shoots/explant	Mean length of axillary shoots (cm)
0.00	0.00	2.0^b	1.30^b	2.3^b	1.39^b
1.11	0.00	2.7^ab	2.00^ab	2.9^b	2.28^ab
2.22	0.00	2.6^ab	1.70^b	2.9^b	2.06^ab
4.48	0.00	2.0^b	1.43^b	2.3^b	1.50^b
0.00	1.16	3.7^a	2.91^a	4.3^a	3.00^a
0.00	2.33	3.2^ab	2.10^ab	4.0^a	2.33^ab
0.00	4.65	2.2^b	1.70^b	2.4^b	2.10^ab

Means in the same column with different letters are statistically significantly different at p ≤ 0.05