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Table 1 Effect of cytokinins in combination with NAA on shoot proliferation from shoot tip explants of Tricholepis roylei inoculated in half-strength MS medium after 6 weeks of culture

From: Micropropagation of Tricholepis roylei Hook. F.—a point endemic species of the Western Himalaya

Plant growth regulators (μM) Explants with shoots (%) No. of shoots per explant Shoot length (cm)
MS0 - - -
BAP
 2.2 18.09 5.37 ± 0.9j 1.9 ± 0.2h
 3.1 36.87 10.08 ± 1.2h 3.1 ± 0.4f
 4.4 68.15 15.48 ± 1.6f 3.4 ± 0.47e
 6.6 59.04 13.26 ± 1.2f 3.2 ± 0.4e
TDZ
 2.27 17.58 5.27 ± 0.8j 1.8 ± 0.4h
 3.18 35.47 10.00 ± 1.1h 2.8 ± 0.2g
 4.54 51.27 13.67 ± 1.3f 3.3 ± 0.2e
 6.8 46.38 11.48 ± 1.0g 3.1 ± 0.2f
Kinetin
 3.25 13.48 4.98 ± 0.8j 1.7 ± 0.6i
 4.6 34.23 8.89 ± 1.1i 2.7 ± 0.3g
 6.92 46.29 12.55 ± 1.3g 3.3 ± 0.2e
 9.3 41.85 11.07 ± 1.2h 3.0 ± 0.15f
BAP+NAA
 4.4 + 0.53 82.23 17.32 ± 1.4e 4.1 ± 0.6c
 4.4 + 1.06 98.89 28.42 ± 2.1a 4.4 ± 0.9a
 4.4 + 1.59 95.26 24.55 ± 2.0b 4.3 ± 0.8b
TDZ+NAA
 4.5 + 0.53 74.82 16.89 ± 1.4e 4.1 ± 0.6c
 4.5 + 1.06 89.01 25.01 ± 2.0b 4.3 ± 0.8b
 4.5 + 1.59 79.72 23.36 ± 1.9c 4.2 ± 0.6c
Kinetin+NAA
 6.9 + 0.53 68.86 14.44 ± 1.5f 3.9 ± 0.5d
 6.9 + 1.06 80.82 24.18 ± 2.0b 4.2 ± 0.6c
 6.9 + 1.59 77.66 21.05 ± 1.8d 4.1 ± 0.6c
  1. #Data are presented as the mean ±SD. Means followed by different letter within columns indicate significant differences at p ≤ 0.05