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Table 1 Effect of cytokinins in combination with NAA on shoot proliferation from shoot tip explants of Tricholepis roylei inoculated in half-strength MS medium after 6 weeks of culture

From: Micropropagation of Tricholepis roylei Hook. F.—a point endemic species of the Western Himalaya

Plant growth regulators (μM)

Explants with shoots (%)

No. of shoots per explant

Shoot length (cm)

MS0

-

-

-

BAP

 2.2

18.09

5.37 ± 0.9j

1.9 ± 0.2h

 3.1

36.87

10.08 ± 1.2h

3.1 ± 0.4f

 4.4

68.15

15.48 ± 1.6f

3.4 ± 0.47e

 6.6

59.04

13.26 ± 1.2f

3.2 ± 0.4e

TDZ

 2.27

17.58

5.27 ± 0.8j

1.8 ± 0.4h

 3.18

35.47

10.00 ± 1.1h

2.8 ± 0.2g

 4.54

51.27

13.67 ± 1.3f

3.3 ± 0.2e

 6.8

46.38

11.48 ± 1.0g

3.1 ± 0.2f

Kinetin

 3.25

13.48

4.98 ± 0.8j

1.7 ± 0.6i

 4.6

34.23

8.89 ± 1.1i

2.7 ± 0.3g

 6.92

46.29

12.55 ± 1.3g

3.3 ± 0.2e

 9.3

41.85

11.07 ± 1.2h

3.0 ± 0.15f

BAP+NAA

 4.4 + 0.53

82.23

17.32 ± 1.4e

4.1 ± 0.6c

 4.4 + 1.06

98.89

28.42 ± 2.1a

4.4 ± 0.9a

 4.4 + 1.59

95.26

24.55 ± 2.0b

4.3 ± 0.8b

TDZ+NAA

 4.5 + 0.53

74.82

16.89 ± 1.4e

4.1 ± 0.6c

 4.5 + 1.06

89.01

25.01 ± 2.0b

4.3 ± 0.8b

 4.5 + 1.59

79.72

23.36 ± 1.9c

4.2 ± 0.6c

Kinetin+NAA

 6.9 + 0.53

68.86

14.44 ± 1.5f

3.9 ± 0.5d

 6.9 + 1.06

80.82

24.18 ± 2.0b

4.2 ± 0.6c

 6.9 + 1.59

77.66

21.05 ± 1.8d

4.1 ± 0.6c

  1. #Data are presented as the mean ±SD. Means followed by different letter within columns indicate significant differences at p ≤ 0.05