Fig. 2
From: Micropropagation of Tricholepis roylei Hook. F.—a point endemic species of the Western Himalaya
Micropropagation of Tricholepis roylei. a, b Initiation of shoots from shoot tip explants after 1 week of incubation in half-strength MS medium enriched with BAP (4.4 μM) plus NAA (1.0 μM). c Proliferation of shoots in half-strength MS medium containing BAP (4.4 μM) plus NAA (1.0 μM). d 6-week-old shoots shifted to half-strength rooting medium. e Initiation of roots after 3 weeks of incubation in half-strength MS medium containing IBA (4.9 μM). f, g Plantlet with fully developed roots after 8 weeks of incubation. h Hardening of plantlets in plastic bags containing sand. i Fully acclimatized plants transferred to pots containing compost-enriched soil after 4 months. (Bars represent 2 cm)