Table 1 Multiple alignment of As_GH cDNA sequence with GenBank sheep database
Current Breed | Codon | Residue/position | Codon | Residue/position | GenBank database |
|---|---|---|---|---|---|
Assaf sheep | ATGATG | Met1Met2 | ATG | Met1 | Ossimi sheep (Egypt) |
GCT | Ala3 | a12a13g14 | Lys2 | ||
C24CC | Pro6 | a21CC | Thr5 | ||
A51CC | Thr15 | g48CC | Ala14 | ||
CAA155 | Gln75 | CAg152 | Gln74 | ||
TCC251 | Ser81 | TCt248 | Ser80 | ||
CCA363 | Pro85 | CCg260 | Pro84 | ||
CTT332 | Leu108 | CTc329 | Leu107 | ||
C387TG | Leu127 | T384TG | Leu126 | ||
GT475T476 | Val156 | Gg472c473 | Gly155 | ||
GAT542 | Asp178 | GAc539 | Asp177 | ||
GA545G | Asp179 | Gc542G | Asp178 | ||
CAC599 | His197 | CAt593 | His195 | ||
TGT632 | Cys208 | TGc626 | Cys205 | ||
CG634C635 | Arg209 | Ca628g629 | Gln207 | ||
TTC662 | Phe218 | TTt656 | Phe216 | ||
T663AA | Stop codon219 | a657AA | Lys217 | ||
G111GC | Gly35 | A25GC | Ser5 | Afghani breed (Pakistan) | |
CAA161 | Gln49 | Cag69 | Gln19 | ||
GAG191 | Glu59 | GAa99 | Glu29 | ||
CCA269 | Pro85 | CCg177 | Pro55 | ||
ACG278 | Thr88 | Aca186 | Thr186 | ||
AAT287 | Asn91 | Aac195 | Asn61 | ||
TCA305 | Ser97 | TCg213 | Ser67 | ||
CTT338 | Leu 108 | CTc246 | Leu77 | ||
CTG393 | Leu127 | T301TG | Leu97 | ||
GAT479 | Asp155 | GAc390 | Asp125 | ||
GT481T482 | Val156 | Gg389c390 | Gly126 | ||
GAT548 | Asp178 | GAc456 | Asp148 | ||
GAT551 | Asp179 | GAc459 | Asp149 | ||
AAG608 | Lys198 | AAa513 | Lys167 | ||
A624GG | Arg204 | C529GG | Arg173 | ||
TGT638 | Cys208 | TGc543 | Cys177 | ||
C54TG | Leu16 | a46TG | Met16 | Kazakh sheep (China) | |
CCa263 | Pro85 | CCg255 | Pro85 | ||
GAT542 | Asp178 | GAc534 | Asp178 | ||
GAT545 | Asp179 | GAc537 | Asp179 | ||
CCa263 | Pro85 | CCg177 | Pro55 | Latti sheep (Pakistan) | |
T510AT | Tyr168 | C424AT | His138 | ||
GA469A | Glu154 | Gg475A | Gly154 | Tibetan sheep (China) | |
AC526A | Thr173 | Ag518A | Arg173 | ||
TCC251 | Ser81 | TCt254 | Ser81 | China sheep | |
GGC89 | Gly89 | GGa278 | Gly89 | ||
CGT416 | Arg134 | CGa413 | Arg134 | ||
TAC563 | Tyr185 | Tat566 | Tyr185 | ||
A637G 638G639 | Arg194 | A distinct mutation, it is absent in sheep or goat breeds | |||