Fig. 3

Electrogram of SDS-polyacrylamide gel electrophoresis of the storage protein of the aerial tuber of D. bulbifera. PAGE was carried out in the presence of SDS using the discontinuous Tris-glycine buffer system. The separating gel was 10%, and stacking gel was 3.5% acrylamide. Other conditions were as described in text. Lane A: standard proteins; B: gel filtration pooled fractions; C: ion exchange pooled fractions; D: ammonium sulphate precipitate; E: crude extract