Fig. 2
From: Double lysis: an integrative time-saving method yielding high-quality RNA from strawberry
Integrity of RNA isolated from strawberry leaves a and fruits b assayed by electrophoretic separation using different extraction protocols: double-lysis method (left), conventional Trizol extraction and extraction by the Direct-zol kit. c Agarose gel electrophoresis showing the migration of RT-PCR product of a fragment of FaGAPDH2 spanning an intron. The expected size of the product is 200 bp in case of a genomic DNA template, and 91 bp in case of a cDNA template, either Direct-zol or Trizol samples are from leaves RNA. Templates obtained from RNA extracted by the double-lysis method from fruits (Fr) and leaves (Lv) are compared to templates from standard Trizol extraction or the spin-column based Direct-zol kit. Gene RulerTM 100 bp plus DNA ladder as M