Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH)

Journal of Genetic Engineering and Biotechnology

Table 2 Statistical analysis of PBD showing coefficient values, main effect, t and p values, and confidence level % for each variable affecting protease with keratinolytic activity production

Variables	Coefficients	Main effect	Standard error	t Stat	p value	Confidence level (%)
Intercept	159.5688		4.62989	34.4648	0.018466363
NH₄Cl	104.7925	209.5849	5.57633	18.7923	0.033844614	96.61554
Feather	15.45379	30.90757	5.35089	2.88807	0.212205337	78.77947
KH₂PO₄	− 41.1885	− 82.37706	5.58779	− 7.3711	0.085842193	91.41578
K₂HPO₄	− 84.9739	− 169.9478	5.59022	− 15.200	0.04182138	95.81786
Yeast extract	100.5857	201.1713	5.60285	17.9525	0.035424603	96.45754
NaCl	− 6.27788	− 12.55575	5.27715	− 1.1896	0.445003112	55.49969
MgSO₄.7H₂O	81.80768	163.6153	5.50027	14.8733	0.042738313	95.72617
CaCl₂	− 64.1394	− 128.2788	5.50027	− 11.661	0.054460083	94.55399
CuSO₄	− 79.1459	− 158.2917	5.27715	− 14.9978	0.042384761	95.76152
MnSO₄	− 77.9249	− 155.817	5.60285	− 13.9081	0.045694769	95.43052
NaNO₃	95.37533	190.7506	5.59022	17.0610	0.0372715	96.27285
Temp.	− 69.0769	− 138.1537	5.58779	− 12.3621	0.051385815	94.86142
pH	80.68165	161.3632	5.35089	15.0781	0.042159573	95.78404
Inoculum size	99.77608	199.5521	5.57633	17.8927	0.035542747	96.44573