The present and potential future methods for delivering CRISPR/Cas9 components in plants

Sandhya, Dulam; Jogam, Phanikanth; Allini, Venkateswar Rao; Abbagani, Sadanandam; Alok, Anshu

doi:10.1186/s43141-020-00036-8

Journal of Genetic Engineering and Biotechnology

Table 3 Agrobacterium-mediated delivery of CRISPR/Cas9 components in different plant species

From: The present and potential future methods for delivering CRISPR/Cas9 components in plants

Plant name	CRISPR/Cas9 vector	Selectable marker	Strain	Target genes	Reference
Arabidopsis thaliana	pUC119-RCS	Marker free	GV3101	AtPDS3, AtFLS2, RACK1b, RACK1c	[41]
Arabidopsis thaliana	pCAMBIA1300	HptII	GV3101	BRI1, GAI, JAZ1	[42]
Banana	pRGEB31	HptII	AGL1	RAS-PDS	[13]
Banana	pRGEB31	HptII	AGL1	LCYε	[43]
Citrus sinensis	pCas9-GN	NptII	LBA4404	CsWRKY22	[44]
Cucumis sativum	pRCS	NptII	EHA105	eIF4E, eIF(iso)4E	[45]
Glycine max	p201N Cas9	NptII	K599	GFP transgene	[46]
Kiwi fruit	pHLW-sgRNA-Cas9-AtU6-1, pPTG-sgRNA-Cas9-U6-1	NptII	EHA105	AcPDS	[47]
Lotus japonicus	pCAMBIA1300	HptII	EHA105	LjLb1, LjLb2, LjLb3, LjSYMRK	[48]
Marchantia polymorpha	pMpGE013 and pMpGE014	HptII	–	MpARF1	[49]
Medicago trancatula	pMDC32-AtU6-26	HptII	ARqual	GUS	[50]
Medicago truncatula	pFGC5941	Bar	–	MtPDS	[51]
Nicotiana benthamaina	pICH86966	–	AGL1	NbPDS, PDS	[14]
Nicotiana benthamaina	pUC19, pKQ334	HptII	GV3101	NbPDS3, NbIspH	[52]
Nicotiana tabaccum	pORE	NptII	LBA4404	NtPDS, NtPDR6	[53]
Oryza sativum	VK005	HptII	EHA105	ISA1	[54]
Populous tomentosa	pYLCRIPSR/Cas9, pUC18	HptII	–	PtoPDS	[55]
Salvia miltiorrhiza	pCAMBIA1300	HptII	C58C1	SmRAS	[56]
Solanum lycopersicum	pYLCRISPR/Cas9	HptII	–	SGR1, LCY-E, Blc, LCY-B1, LCY-B2	[57]
Solanum lycopersicum	pENTR-sgRNA: pMR290/Cas9	NptII	EHA105	SlCCD8	[58]
Solanum tuberosum	pMDC32	HptII	–	StALS1	[59]
Sorghum bicolor	pVS1 binary vector derived from pLH7500	NptII	Y158	DsRED2	[20]
Triticum aesituvam	pBI121	NptII	GV3101	Inox, PDS	[14]
Zea mays	pMCG1005	Bar	EHA101	Argonaute 18, Dihydroflavonol-4-reductase	[60]

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