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Table 3 Agrobacterium-mediated delivery of CRISPR/Cas9 components in different plant species

From: The present and potential future methods for delivering CRISPR/Cas9 components in plants

Plant name CRISPR/Cas9 vector Selectable marker Strain Target genes Reference
Arabidopsis thaliana pUC119-RCS Marker free GV3101 AtPDS3, AtFLS2, RACK1b, RACK1c [41]
Arabidopsis thaliana pCAMBIA1300 HptII GV3101 BRI1, GAI, JAZ1 [42]
Banana pRGEB31 HptII AGL1 RAS-PDS [13]
Banana pRGEB31 HptII AGL1 LCYε [43]
Citrus sinensis pCas9-GN NptII LBA4404 CsWRKY22 [44]
Cucumis sativum pRCS NptII EHA105 eIF4E, eIF(iso)4E [45]
Glycine max p201N Cas9 NptII K599 GFP transgene [46]
Kiwi fruit pHLW-sgRNA-Cas9-AtU6-1, pPTG-sgRNA-Cas9-U6-1 NptII EHA105 AcPDS [47]
Lotus japonicus pCAMBIA1300 HptII EHA105 LjLb1, LjLb2, LjLb3, LjSYMRK [48]
Marchantia polymorpha pMpGE013 and pMpGE014 HptII MpARF1 [49]
Medicago trancatula pMDC32-AtU6-26 HptII ARqual GUS [50]
Medicago truncatula pFGC5941 Bar MtPDS [51]
Nicotiana benthamaina pICH86966 AGL1 NbPDS, PDS [14]
Nicotiana benthamaina pUC19, pKQ334 HptII GV3101 NbPDS3, NbIspH [52]
Nicotiana tabaccum pORE NptII LBA4404 NtPDS, NtPDR6 [53]
Oryza sativum VK005 HptII EHA105 ISA1 [54]
Populous tomentosa pYLCRIPSR/Cas9, pUC18 HptII PtoPDS [55]
Salvia miltiorrhiza pCAMBIA1300 HptII C58C1 SmRAS [56]
Solanum lycopersicum pYLCRISPR/Cas9 HptII SGR1, LCY-E, Blc, LCY-B1, LCY-B2 [57]
Solanum lycopersicum pENTR-sgRNA: pMR290/Cas9 NptII EHA105 SlCCD8 [58]
Solanum tuberosum pMDC32 HptII StALS1 [59]
Sorghum bicolor pVS1 binary vector derived from pLH7500 NptII Y158 DsRED2 [20]
Triticum aesituvam pBI121 NptII GV3101 Inox, PDS [14]
Zea mays pMCG1005 Bar EHA101 Argonaute 18, Dihydroflavonol-4-reductase [60]
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