Table 2 Particle bombardment method for CRISPR/Cas9 component delivery
From: The present and potential future methods for delivering CRISPR/Cas9 components in plants
Plant name | CRISPR/Cas9 vector or RNP complex | Selectable marker | Target genes | Reference |
|---|---|---|---|---|
Glycine max | QC810 and RTW830, QC799 and RTW831 | HptII | DD20, DD43 | [33] |
Hordeum vulgare | pcas9:sgRNA | HptII | ENGase | [34] |
Oryza sativum | pCam1300-CRISPR-B | HptII | crtI, ZmPsy | [35] |
Oryza sativum | CRISPR-RNP complex | HptII | OsPDS1 | [36] |
Oryza sativum | pJIT163-2NLSCas9 | HptII | OsPDS, OsBADH2 | [21] |
Oryza sativum | pOsU3-sgRNA, pJIT163-2NLSCas9 | HptII | OsPDS, OsDEP1 | [37] |
Triticum aesituvam | pJIT163-Ubi | bar | TaMLO-A1, TaMLO-B1, TaMLO-D1 | [38] |
Zea mays | pSB11-Ubi:Cas9 | Pat | LIG1, Ms26, Ms45, ALS1, ALS2 | [32] |